RPS6KB2 antibody (pSer447, pThr444)

Catalog No. ABIN702850

Product Details anti-RPS6KB2 Antibody

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Binding Specificity: pSer447, pThr444

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p70 S6 Kinase Beta around the phosphorylation site of Thr444/Ser447

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KB2

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Alternative Name: p70 S6 Kinase Beta + (RPS6KB2 Products)

Synonyms: KLS antibody, P70-beta antibody, P70-beta-1 antibody, P70-beta-2 antibody, S6K-beta2 antibody, S6K2 antibody, SRK antibody, STK14B antibody, p70(S6K)-beta antibody, p70S6Kb antibody, 70kDa antibody, ribosomal protein S6 kinase B2 antibody, ribosomal protein S6 kinase, polypeptide 2 antibody, RPS6KB2 antibody, Rps6kb2 antibody

Background:

Synonyms: p70 S6 KinaseThr444 + Ser447, P70 S6 Kinase beta phospho T444 + S447, 70 kDa ribosomal protein S6 kinase 2, p70 beta, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6KB, p70 S6Kbeta, p70S6K beta, p70-beta, p70-S6KB, p70S6Kb, Ribosomal protein S6 kinase 70 kDa, polypeptide 2, Ribosomal protein S6 kinase beta 2, Ribosomal protein S6 kinase beta-2, RPS6KB2, S6 kinase related kinase, S6 kinase-related kinase, S6K beta 2, S6K beta, S6K-beta, S6K2, Serine/threonine protein kinase 14 beta, Serine/threonine-protein kinase 14 beta, SRK, STK14B, KS6B1_HUMAN.

Background: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway