Iba1 antibody (Intracellular)

Catalog No. ABIN7042910

Product Details anti-Iba1 Antibody

Target: Iba1 (AIF1) (Allograft Inflammatory Factor 1 (AIF1))

Binding Specificity: AA 40-53, Intracellular

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Anti-IBA1/AIF1 Antibody (ABIN7042910, ABIN7044214 and ABIN7044215)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IBA1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Iba1

Target: Iba1 (AIF1) (Allograft Inflammatory Factor 1 (AIF1))

Alternative Name: IBA1/AIF1 (AIF1 Products)

Synonyms: AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody

Background: Alternative names: Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor

Gene ID: 29427

NCBI Accession: NM_001318970

UniProt: P55009

Pathways: Smooth Muscle Cell Migration