ACCN4 antibody (Intracellular)

Catalog No. ABIN7042918

This Rabbit Polyclonal antibody specifically detects ACCN4 in WB. It exhibits reactivity toward Rat.

Quick Overview for ACCN4 antibody (Intracellular) (ABIN7042918)

Target: ACCN4 (Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACCN4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACCN4 Antibody

Binding Specificity: AA 7-26, Intracellular

Purpose: A Rabbit Polyclonal Antibody to ASIC4 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-ASIC4 Antibody is directed against an epitope of rat ASIC4. Anti-ASIC4 Antibody (ABIN7042918 and ABIN7045238) can be used in western blot analysis. It has been designed to recognize ASIC4 from rat, human and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234712)

Blocking Peptide: (ABIN7234712)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ACCN4

Target: ACCN4 (Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4))

Alternative Name: ASIC4

Background: Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4,Acid-sensing ion channels (ASICs) are Na+ channels activated by external protons. ASIC4 is a member of the ASIC family that includes four additional members: ASIC1, ASIC2, ASIC3 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional ASIC channel is composed of 4 subunits, which can form either a homo- or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed.Expression of the ASIC4 protein has been detected in the brain, pituitary gland, spinal cord and inner ear. The functional significance of ASIC4 expression in these tissues still awaits clarification.

Alternative names: ASIC4, Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4

Gene ID: 63882

NCBI Accession: NM_018674

UniProt: Q9QYV9