GLRB antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7886414

This Rabbit Polyclonal antibody specifically detects GLRB in WB. It exhibits reactivity toward Rat.

Quick Overview for GLRB antibody (2nd Cytoplasmic Loop) (ABIN7886414)

Target: GLRB (Glycine Receptor, beta (GLRB))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLRB Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 394-405

Purpose: A Rabbit Polyclonal Antibody to Glycine Receptor β

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Glycine Receptor beta Antibody is directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (ABIN7043260, ABIN7044402 and ABIN7044403) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRbeta

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235724)

Blocking Peptide: (ABIN7235724)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GLRB

Target: GLRB (Glycine Receptor, beta (GLRB))

Alternative Name: GLRB

Background:

Synonyms: Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ

Description: The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.

Gene ID: 25456

NCBI Accession: NM_000824

UniProt: P20781