HCRTR1 antibody (3rd Intracellular Loop) (Atto 488)

Catalog No. ABIN7043286

Product Details anti-HCRTR1 Antibody (Atto 488)

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Binding Specificity: 3rd Intracellular Loop, AA 256-269

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCRTR1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples. \nAnti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#ABIN7043286) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCRTR1

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Alternative Name: Orexin Receptor 1 (HCRTR1 Products)

Synonyms: OX1R antibody, Ox1r antibody, Hctr1 antibody, HCRTR1 antibody, hypocretin receptor 1 antibody, hypocretin (orexin) receptor 1 antibody, HCRTR1 antibody, Hcrtr1 antibody

Background: Alternative names: Orexin Receptor 1, OX1R, Hypocretin receptor type 1, HCRTR1

Gene ID: 25593

NCBI Accession: NM_001525

UniProt: P56718

Pathways: Feeding Behaviour