KCNJ11 antibody (Intracellular)

Catalog No. ABIN7043472

Product Details anti-KCNJ11 Antibody

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Binding Specificity: AA 372-385, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This KCNJ11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Guinea Pig Polyclonal Antibody to Kir6.2 Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody is specific for Kir6.2 and does not cross-react with Kir6.1.

Predicted Reactivity: Mouse - identical, human - 12,14 amino acid residues identical

Characteristics: Guinea pig Anti-Kir6.2 Antibody is directed against an epitope of rat Kir6.2. Guinea pig Anti-Kir6.2 Antibody raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir6.2 Antibody (ABIN7043473, ABIN7044922 and ABIN7044923) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: (ABIN7236368)

Blocking Peptide: (ABIN7236368)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Alternative Name: KCNJ11 (KCNJ11 Products)

Background: Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3,Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides. The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle. The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit. The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.

Alternative names: Kir6.2, Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3

Gene ID: 83535

NCBI Accession: NM_000525

UniProt: P70673

Pathways: Negative Regulation of Hormone Secretion