P2RX7 antibody (Intracellular)

Catalog No. ABIN7043576

The Rabbit Polyclonal anti-P2RX7 antibody has been validated for WB, IHC, IF, IP and IC. It is suitable to detect P2RX7 in samples from Rat.

Quick Overview for P2RX7 antibody (Intracellular) (ABIN7043576)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-P2RX7 Antibody

Binding Specificity: AA 576-595, Intracellular

Purpose: A Rabbit Polyclonal Antibody to P2X7 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 16,20 amino acid residues identical,Mouse - 18

Characteristics: Anti-P2X7 Receptor Antibody (ABIN7043576, ABIN7045076 and ABIN7045077) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2X7 purinergic receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|IFC

Negative Control: (ABIN7236672)

Blocking Peptide: (ABIN7236672)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Alternative Name: P2RX7

Background: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor,The P2X7 purinergic receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family consists of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells and microglia.The P2X7 receptor mediates the release of proinflamatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3

Alternative names: P2X7 Receptor, Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Gene ID: 29665

NCBI Accession: NM_002562

UniProt: Q64663

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis