P2RX7 antibody (Extracellular Loop) (Atto 488)

Catalog No. ABIN7043579

Product Details anti-P2RX7 Antibody (Atto 488)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Binding Specificity: AA 136-152, Extracellular Loop

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX7 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Live Cell Imaging (LCI)

Characteristics: Anti-P2X7 Receptor (extracellular) Antibody (ABIN7043578, ABIN7045080 and ABIN7045081)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples. \nAnti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#ABIN7043579) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Alternative Name: P2X7 Receptor (P2RX7 Products)

Synonyms: p2xr7 antibody, P2RX7 antibody, P2X7 antibody, AI467586 antibody, P2X(7) antibody, P2X7R antibody, p2x7 antibody, purinergic receptor P2X 7 antibody, purinergic receptor P2X, ligand-gated ion channel, 7 antibody, P2X purinoceptor 7 antibody, P2RX7 antibody, p2rx7 antibody, LOC100458463 antibody, P2rx7 antibody

Background: Alternative names: P2X7 Receptor, Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Gene ID: 18439

NCBI Accession: NM_002562

UniProt: Q9Z1M0

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis