SEMA4D/CD100 antibody (Extracellular, N-Term)

Catalog No. ABIN7043611

Product Details anti-SEMA4D/CD100 Antibody

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Binding Specificity: AA 80-93, Extracellular, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Characteristics: Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody (ABIN7043611, ABIN7045298 and ABIN7045299)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SEMA4D from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKQHEVYWKVSEDK, corresponding to amino acid residues 80-93 of mouse SEMA4D

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: Semaphorin 4D (SEMA4D) (SEMA4D Products)

Synonyms: C9orf164 antibody, CD100 antibody, M-sema-G antibody, SEMAJ antibody, coll-4 antibody, Semacl2 antibody, Semaj antibody, Semcl2 antibody, COLL-4 antibody, semaphorin 4D antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D antibody, Sema4d antibody, SEMA4D antibody

Background: Alternative names: Semaphorin 4D (SEMA4D), CD100, Semaphorin-J

Gene ID: 20354

NCBI Accession: NM_006378

UniProt: O09126

Pathways: Regulation of Cell Size