IL-3 antibody (AA 81-152) (HRP)

Catalog No. ABIN706715

Product Details anti-IL-3 Antibody (HRP)

Target: IL-3 (Interleukin 3 (IL-3))

Binding Specificity: AA 81-152

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IL-3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3 (IL-3 Products)

Synonyms: BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody

Background:

Synonyms: Burst promoting activity, Colony stimulating factor multiple, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, IL3, IL3_HUMAN, Interleukin 3 colony stimulating factor, multiple, IL 3, Interleukin 3, Mast cell growth factor, MCGF, MGC79398, MGC79399, Multi CSF, Multilineage colony stimulating factor, Multipotential colony stimulating factor, P cell stimulating factor, Thy1 inducing factor, BPA, HCGF, Il 3, Interleukin-3, Mast cell growth factor, MCGF, Multi CSF, OTTHUMP00000065963, P-cell-stimulating factor, MCGF, MGC79399, Csfmu, Hematopoietic growth factor, IL3B, Interleukin 3 beta, Interleukin 3 precursor, Mast-cell growth factor, MGC79398, MULTI-CSF, Multilineage colony stimulating factor, Multipotential colony-stimulating factor, P cell stimulating factor, PSF, RP23-309E16.5.

Background: The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Gene ID: 3562

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy