NDFIP1 antibody

Catalog No. ABIN7116768

Product Details anti-NDFIP1 Antibody

Target: NDFIP1 (Nedd4 Family Interacting Protein 1 (NDFIP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDFIP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: Nedd4 family interacting protein 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for NDFIP1

Target: NDFIP1 (Nedd4 Family Interacting Protein 1 (NDFIP1))

Alternative Name: NDFIP1 (NDFIP1 Products)

Synonyms: zgc:55546 antibody, wu:fc17e11 antibody, n4wbp5 antibody, MGC108163 antibody, ndfip1 antibody, NDFIP1 antibody, N4WBP5 antibody, 0610010M22Rik antibody, N4wbp5 antibody, Nedd4 family interacting protein 1 antibody, Nedd4 WW domain-binding protein 5 antibody, ndfip1 antibody, NDFIP1 antibody, n4wbp5 antibody, Ndfip1 antibody

Background: Synonyms:N4WBP5 Background:Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation(By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity(PubMed:19706893). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate(PubMed:20534535). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1(PubMed:25631046).

Molecular Weight: 30 kDa

Gene ID: 80762

UniProt: Q9BT67

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity