NUDT16 antibody

Catalog No. ABIN7117078

Product Details anti-NUDT16 Antibody

Target: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUDT16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: nudix(nucleoside diphosphate linked moiety X)-type motif 16

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:4000, IP: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for NUDT16

Target: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Alternative Name: NUDT16 (NUDT16 Products)

Synonyms: RGD1311387 antibody, 2310041H06Rik antibody, 2810047L04Rik antibody, 2900006H04Rik antibody, AI851783 antibody, x29 antibody, nudix hydrolase 16 antibody, nudix (nucleoside diphosphate linked moiety X)-type motif 16 antibody, nudix hydrolase 16 L homeolog antibody, Nudt16 antibody, NUDT16 antibody, nudt16.L antibody

Background: Synonyms:Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme Background:RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate(m7GpppG). Also hydrolysis m7G-and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA, metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase, hydrolyzes the non-canonical purine nucleotides inosine diphosphate(IDP) and deoxyinosine diphosphate(dITP) as well as guanosine diphosphate(GDP), deoxyguanosine diphosphate(dGDP), xanthine diphosphate(XDP), inosine triphosphate(ITP) and deoxyinosine triphosphate(ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy-and ribose forms(PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP(PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions(PubMed:20385596).

Molecular Weight: 21 kDa

Gene ID: 131870

UniProt: Q96DE0

Pathways: Positive Regulation of Response to DNA Damage Stimulus