2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
Background
Synonyms: Background:Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone(By similarity).