Glycogen Synthase Kinase 3 (GSK3) (pTyr216), (pTyr279) antibody

Catalog No. ABIN7138967

Product Details

Target: Glycogen Synthase Kinase 3 (GSK3)

Binding Specificity: pTyr216, pTyr279

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta .

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: Glycogen Synthase Kinase 3 (GSK3)

Alternative Name: GSK3A/GSK3B (GSK3 Products)

Synonyms: GSK3 antibody, GSK-3 antibody, C86142 antibody, GSK-3beta antibody, 7330414F15Rik antibody, 8430431H08Rik antibody, CG2621 antibody, DMSGG3 antibody, DMZ3K25Z antibody, Dm Zw3 antibody, Dmel\CG2621 antibody, Dmsgg3 antibody, EG:155E2.3 antibody, EG:BACR7C10.8 antibody, GSK antibody, GSK-3B antibody, GSK-3betaSgg antibody, GSK3-beta antibody, GSK3A antibody, GSK3Beta antibody, GSK3beta antibody, GSK3beta/SGG antibody, GSK3betagr antibody, GSKbeta antibody, Gsk-3 antibody, Gsk-3beta antibody, Gsk3 antibody, SGG antibody, SGG/GSK3b antibody, Sgg antibody, Sgg/GSK-3b antibody, Sgg/Zw3/GSK3 antibody, ZW-3 antibody, ZW3 antibody, Zw-3 antibody, Zw3 antibody, Zw3-sh antibody, Zw3/GSK3 antibody, Zw3[sgg] antibody, anon-WO03040301.254 antibody, gsk3 antibody, l(1)3Ba antibody, l(1)G0055 antibody, l(1)G0183 antibody, l(1)G0263 antibody, l(1)G0335 antibody, l(1)zw3 antibody, sgg-zw3 antibody, sgg/zw3 antibody, sgg10 antibody, sgg39 antibody, sgg46 antibody, zw-3 antibody, zw3 antibody, zw3(sgg) antibody, zw3/GSK3beta antibody, zw3/sgg antibody, zw3[sgg] antibody, zw[3] antibody, glycogen synthase kinase 3 beta antibody, shaggy antibody, Gsk3b antibody, sgg antibody

Background:

Background: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13, 137(1-2): 193-201 Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02, 316(2): 411-415 Fan G, et al. J Biol Chem 2003 Dec 26, 278(52): 52432-52436 Liao X, et al. Mol Cancer Ther 2003 Nov, 2(11): 1215-1222

Aliases: Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta

UniProt: P49840, P49841