POLD3 antibody (AA 203-275) (Biotin)

Catalog No. ABIN7150236

Product Details anti-POLD3 Antibody (Biotin)

Target: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Binding Specificity: AA 203-275

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD3 antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human DNA polymerase delta subunit 3 protein (203-275AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for POLD3

Target: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Alternative Name: POLD3 (POLD3 Products)

Synonyms: zgc:136471 antibody, P66 antibody, P68 antibody, 2410142G14Rik antibody, C85233 antibody, polymerase (DNA-directed), delta 3, accessory subunit L homeolog antibody, polymerase (DNA-directed), delta 3, accessory subunit antibody, DNA polymerase delta 3, accessory subunit antibody, pold3.L antibody, pold3 antibody, POLD3 antibody, Pold3 antibody

Background:

Background: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).

Aliases: DNA polymerase delta subunit 3 antibody, DNA polymerase delta subunit p66 antibody, DNA polymerase delta, subunit 3 antibody, DNA polymerase subunit delta p66 antibody, DPOD3_HUMAN antibody, KIAA0039 antibody, P66 antibody, P68 antibody, Pol delta C subunit (p66) antibody, Pold3 antibody, Polymerase (DNA directed), delta 3 antibody, Polymerase (DNA-directed), delta 3, accessory subunit antibody, PPP1R128 antibody, Protein phosphatase 1, regulatory subunit 128 antibody

UniProt: Q15054

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA