14-3-3 Pan (acLys49), (acLys51) antibody

Catalog No. ABIN7226752

The Rabbit Polyclonal anti-not_set antibody has been validated for WB and ELISA. It is suitable to detect not_set in samples from Human, Mouse and Rat.

Quick Overview for 14-3-3 Pan (acLys49), (acLys51) antibody (ABIN7226752)

Target: 14-3-3 Pan

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: acLys49, acLys51

Purpose: 14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody

Specificity: Acetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human 14-3-3-pan around the acetylation site of K51

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: 14-3-3 Pan

Alternative Name: 14-3-3-pan

Background: Rabbit Anti-14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody,YWHAB (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta) encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for YWHAB.,14-3-3 protein beta

Gene ID: 7529

UniProt: P31946