Caspase 3 antibody (AA 20-100)

Catalog No. ABIN7212202

The Mouse Monoclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and IF (p).

Quick Overview for Caspase 3 antibody (AA 20-100) (ABIN7212202)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 5H10

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 20-100

Purpose: Mouse Anti-Caspase-3 Mouse Monoclonal Antibody (5H10)

Specificity: Caspase-3 protein detects endogenous levels of CASP3.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide of Caspase-3 at AA range of 20-100

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB: 500-2000,IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: CASP3CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.

Molecular Weight: 17, 32

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation