NFYB antibody (AA 10-90)

Catalog No. ABIN7216048

The Rabbit Polyclonal anti-NFYB antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect NFYB in samples from Human, Mouse and Rat.

Quick Overview for NFYB antibody (AA 10-90) (ABIN7216048)

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFYB Antibody

Binding Specificity: AA 10-90

Purpose: NF-YB Polyclonal Antibody

Specificity: NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human NF-YB at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NF-YB

Background: Rabbit Anti-NF-YB Polyclonal Antibody,NFYB, HAP3, Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB,Nuclear transcription factor Y subunit beta encoded by NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYB product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.,Nuclear transcription factor Y subunit beta

Molecular Weight: observerd band 29kDa

Gene ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha