CHRNA2 antibody (Extracellular, N-Term)

Catalog No. ABIN7886585

The Rabbit Polyclonal anti-CHRNA2 antibody (ABIN7886585) specifically detects CHRNA2 in WB, IHC, IC, IF and LCI. The antibody is reactive with Rat samples.

Quick Overview for CHRNA2 antibody (Extracellular, N-Term) (ABIN7886585)

Target: CHRNA2 (Cholinergic Receptor, Nicotinic, alpha 2 (Neuronal) (CHRNA2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-CHRNA2 Antibody

Binding Specificity: AA 189-201, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2

Sequence: (C)DLEQMERTVD LKD

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Highly specific antibody directed against an extracellular epitope of rat nAChRα2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) (CHRNA2) Antibody (ABIN7581852) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody is specially suited to recognize nAChRα2 in live cells. It has been designed to recognize nAChRα2 from rat mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRalpha2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CHRNA2

Target: CHRNA2 (Cholinergic Receptor, Nicotinic, alpha 2 (Neuronal) (CHRNA2))

Alternative Name: CHRNA2

Background:

Synonyms: nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2

Description: Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and have longer-term effects on gene expression and cellular connections1.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits ('structural' subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2. There are nine α subunits (α2-α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and and β (2-4) subunits. All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5.In humans, a mutant α2 subunit has been identified, which forms nAChRs with increased agonist sensitivity and causes a form of familial epilepsy6. Further, an α2 subunit null mouse model has been used to demonstrate a role for α2 nAChR in nicotine-induced modulation of long-term potentiation in the mouse hippocampal CA1 region, which may underlie some of the cognitive effects of nicotine7.

Gene ID: 170945

UniProt: P12389