GABRB2 antibody (Intracellular)

Catalog No. ABIN7581873

The Rabbit Polyclonal anti-GABRB2 antibody (ABIN7581873) specifically detects GABRB2 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for GABRB2 antibody (Intracellular) (ABIN7581873)

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABRB2 Antibody

Binding Specificity: AA 405-419, Intracellular

Purpose: A Rabbit Polyclonal antibody to GABA(A) β2 Receptor

Sequence: (C)KAGLPRHSFG RNALE

Specificity: Intracellular, 2nd loop.

Predicted Reactivity: Mouse,Human - identical

Characteristics: Anti-GABA(A) β2 Receptor Antibody (ABIN7581873) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) Receptor Beta 2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KAGLPRHSFGRNALE, corresponding to amino acid residues 405 - 419 of rat GABRB2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABRB2

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Alternative Name: GABRB2

Background: Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2,GABA receptors have two main subtypes: GABA(A) and GABA(B) receptors based on their amino acid sequence and structure. The GABA(A) receptors are ionotropic receptors while the GABA(B) receptors are metabotropic G-protein coupled receptors1.GABA(A) receptors are ion channels opened when GABA or its selective agonist muscimol binds to the receptor. In contrast, when the competitive antagonists bicuculline or gabazine binds to the GABA(A) receptors, the GABA-evoked current is decreased. GABA(A) receptors are blocked with picrotoxin, a non-competitive blocker, that blocks the GABA(A) receptor pore. Benzodiazepines and barbiturates are positive allosteric modulators of GABA(A) receptors and can enhance the currents of these channels2-4.To date, 19 GABA(A) receptor subunits have been identified: α 1-6, β 1-3, γ1-3, δ, ε, θ, p and ? 1-34. GABA(A)receptors are heteropentameric complexes formed by combinations of different GABA(A) receptor subunits 4.The major isoform of the GABA(A) receptor in the brain is composed of two α1, two β2, and one γ2 subunits that are encoded by the GABRA1, GABRB2, and GABRG2 genes respectively, accounting for 43 % of the GABA receptors in the mammalian brain 5. It is then not surprising that GABA(A) Receptor Beta 2, has been associated with several neuropsychiatric disorders such as schizophrenia, bipolar disorder, epilepsy, autism spectrum disorder, Alzheimer's disease, frontotemporal dementia and depression 5.

Gene ID: 25451

UniProt: P63138

Pathways: Sensory Perception of Sound, Synaptic Membrane