Recombinant BRAF antibody (V600E) (Biotin)
The Rabbit Monoclonal anti-BRAF antibody has been validated for WB, ELISA, IHC, FACS and ICC. It is suitable to detect BRAF in samples from Human.
Quick Overview for Recombinant BRAF antibody (V600E) (Biotin) (ABIN7670296)
Target
See all BRAF Antibodies
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Antibody Type
Recombinant Antibody
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Conjugate
This BRAF antibody is conjugated to Biotin
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
Clone
RM8
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Binding Specificity
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V600E
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Purpose
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Biotinylated Rabbit monoclonal to BRAF V600E Mutant
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Specificity
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This antibody reacts to the BRAF V600E mutant.
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Cross-Reactivity (Details)
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No cross reactivity with wild type BRAF.
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Purification
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Protein A affinity purified from an animal origin-free culture supernatant
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Immunogen
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A peptide corresponding to BRAF V600E mutant
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Isotype
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IgG
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Application Notes
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Immunohistochemistry (IHC): 2 - 10 μg/mL, Immunocytochemistry (ICC): 2 - 10 μg/mL, WB: 0.5 - 2 μg/mL, ELISA: 0.5 - 2 μg/mL
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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1 mg/mL
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Buffer
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50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
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-20 °C
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Storage Comment
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-20.0°C
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Target
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BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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Alternative Name
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BRAF
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Gene ID
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673
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UniProt
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P15056
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Pathways
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MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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