PFN2 antibody (AA 51-140)

Catalog No. ABIN735728

Product Details anti-PFN2 Antibody

Target: PFN2 (Profilin 2 (PFN2))

Binding Specificity: AA 51-140

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody may detect Profilin 1 due to a 71 % amino acid sequence similarity in the immunogen sequence.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Profilin 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PFN2

Target: PFN2 (Profilin 2 (PFN2))

Alternative Name: Profilin 2 (PFN2 Products)

Background:

Synonyms: Profilin-2, Profilin II, Pfn2

Background: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).

Gene ID: 81531

UniProt: Q9EPC6

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis