FITC antibody
Quick Overview for FITC antibody (ABIN7833249)
Target
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-FITC | fluorescein isothiocyanate (monoclonal, Clone NI 239)
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Cross-Reactivity (Details)
- FITC molecule
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Purification
- Purified IgG
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Immunogen
- Highly purified fluorescein isothiocyanate isomer 1
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Application Notes
- The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (IL)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 0.5 mL of sterile water
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Buffer
- Purified IgG.1 in PBS pH 7.2.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
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- FITC (Fluorescein Isothiocyanate (FITC))
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Alternative Name
- FITC
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Target Type
- Chemical
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Background
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Background: FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Additional information: This product is intended FOR RESEARCH USE ONLY
Target
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