PRKCA beta 2 antibody (pThr638, pThr641)

Catalog No. ABIN744758

Product Details anti-PRKCA beta 2 Antibody

Target: PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))

Binding Specificity: pThr638, pThr641

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKCA beta 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Dog, Mouse, Rat

Predicted Reactivity: Human,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PRKCA beta 2 antibody (ABIN744758)

Edens, Dilsaver, Levy: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Edens, Levy: "cPKC regulates interphase nuclear size during Xenopus development." in: The Journal of cell biology, Vol. 206, Issue 4, pp. 473-83, (2014) (PubMed).

Target Details for PRKCA beta 2

Target: PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))

Abstract: PRKCA beta 2 Products

Background:

Synonyms: AAG6, PKCA, PRKACA, PKC-alpha, Protein kinase C alpha type, PKC-A, PRKCA

Background: Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

Gene ID: 5578

UniProt: P17252