RPS6KA3 antibody (pSer363, pThr359)

Catalog No. ABIN745208

Product Details anti-RPS6KA3 Antibody

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Binding Specificity: pSer363, pThr359

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human p90RSK1 around the phosphorylation site of Thr359/Ser363

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: p90RSK + (RPS6KA3 Products)

Synonyms: HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, CLS antibody, HU-3 antibody, ISPK-1 antibody, MAPKAPK1B antibody, MRX19 antibody, RSK2 antibody, S6K-alpha3 antibody, p90-RSK2 antibody, pp90RSK2 antibody, MPK-9 antibody, Rsk2 antibody, RGD1563860 antibody, id:ibd1103 antibody, rps6ka3 antibody, si:ch211-114c12.12 antibody, wu:fk86e02 antibody, zgc:56006 antibody, zgc:158386 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase A3 antibody, ribosomal protein S6 kinase polypeptide 3 antibody, ribosomal protein S6 kinase a, polypeptide 3a antibody, ribosomal protein S6 kinase, polypeptide 3b antibody, RPS6KA1 antibody, RPS6KA3 antibody, Rps6ka3 antibody, rps6ka3a antibody, rps6ka3b antibody

Background:

Synonyms: p90 RSK1, p90rsk, p90S6K, pp90RSK1, Ribosomal protein S6 kinase 90kD 1, Ribosomal protein S6 kinase 90kD polypeptide 1, Ribosomal protein S6 kinase 90 kDa polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal S6 kinase 1, RPS6K1 alpha, rps6ka, Rps6ka1, RSK 1, RSK 1 p90, RSK, RSK1, S6K alpha 1, 90 kDa ribosomal protein S6 kinase 1, dJ590P13.1 ribosomal protein S6 kinase, 90kD, polypeptide 1, dJ590P13.1, EC 2.7.11.1, HU 1, HU1, MAP kinase activated protein kinase 1a, MAPKAP kinase 1A, MAPKAPK1A, MGC79981, Mitogen-activated protein kinase-activated protein kinase 1A

Background: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4).

Gene ID: 6195

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades