SPHK2 antibody (AA 101-200)

Catalog No. ABIN748613

Product Details anti-SPHK2 Antibody

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Binding Specificity: AA 101-200

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPHK2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SPHK2 antibody (ABIN748613)

Tran, Jersmann, Truong, Hamon, Roscioli, Ween, Pitman, Pitson, Hodge, Reynolds, Hodge: "Disrupted epithelial/macrophage crosstalk via Spinster homologue 2-mediated S1P signaling may drive defective macrophage phagocytic function in COPD." in: PLoS ONE, Vol. 12, Issue 11, pp. e0179577, (2017) (PubMed).

Zhang, Li, Yuan, Zhao: "Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway." in: Experimental and molecular pathology, Vol. 100, Issue 1, pp. 51-8, (2016) (PubMed).

Tani, Tabuchi, Hara: "Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea." in: Neurotoxicity research, Vol. 29, Issue 1, pp. 35-46, (2016) (PubMed).

Tran, Barnawi, Ween, Hamon, Roscioli, Hodge, Reynolds, Pitson, Davies, Haberberger, Hodge: "Cigarette smoke inhibits efferocytosis via deregulation of sphingosine kinase signaling: reversal with exogenous S1P and the S1P analogue FTY720." in: Journal of leukocyte biology, Vol. 100, Issue 1, pp. 195-202, (2016) (PubMed).

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SPHK2 (SPHK2 Products)

Synonyms: CG2159 antibody, CG32484 antibody, Dmel\\CG32484 antibody, SK2 antibody, Sphk2 antibody, sk2 antibody, DKFZp469F224 antibody, SK 2 antibody, SK-2 antibody, SPK 2 antibody, SPK-2 antibody, C76851 antibody, Sphingosine kinase antibody, sphk2 antibody, Sphingosine kinase 2 antibody, sphingosine kinase 2 antibody, sphK2 antibody, Sk2 antibody, SPHK2 antibody, Sphk2 antibody

Background:

Synonyms: SK 2, SK-2, SPK 2, SPK-2, Sphingosine kinase 2, SPHK2

Background: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.

Gene ID: 56848

UniProt: Q9NRA0

Pathways: VEGF Signaling