Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A) (C-Term) antibody

Details for Product No. ABIN768561
Antigen
  • idh3a
  • MGC76128
  • IDH3A
  • zgc:56380
  • zgc:85631
  • BG1
  • 1110003P10Rik
  • 1500012E04Rik
  • AA407078
  • AI316514
  • isocitrate dehydrogenase 3 (NAD+) alpha
  • isocitrate dehydrogenase 3 (NAD(+)) alpha
  • isocitrate dehydrogenase 3 (NAD+) alpha S homeolog
  • idh3a
  • IDH3A
  • idh3a.S
  • Idh3a
Epitope
C-Term
15
5
4
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
64
45
42
8
7
6
5
5
3
3
2
1
1
1
Host
Goat
50
8
6
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
37
21
13
13
10
7
4
1
1
Options
Purpose IDH3A
Immunogen Peptide with sequence DFTEEICRRVKDLD, from the C Terminus of the protein sequence according to NP_005521.1.
Blocking Peptide Blocking peptide for this product available: ABIN2815857
Sequence DFTEEICRRV KDLD
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name IDH3A (IDH3A Antibody Abstract)
Background IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-IDH alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitric dehydrogenase subunit alpha
Molecular Weight 39.6kDa according to NP_005521.1
Gene ID 3419
NCBI Accession NP_005521
Application Notes

DS WB Results: Approx 37 kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38 kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6 kDa according to NP_005521.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Thymus. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A) (C-Term) antibody (ABIN768561) ABIN768561 (0.1 µg/mL) staining of Human (A), Mouse (B) and Rat (C) Spleen lysates (3...
 image for anti-Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A) (C-Term) antibody (ABIN768561) ABIN768561 (0.1 µg/mL) staining of Human Lymph Nodes lysate (35 µg protein in RIPA bu...
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