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NRF2 antibody (AA 445-458)

Verified NFE2L2 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN768601
  • Target See all NRF2 (NFE2L2) Antibodies
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Binding Specificity
    • 23
    • 15
    • 9
    • 8
    • 8
    • 7
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 445-458
    Reactivity
    • 121
    • 57
    • 55
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 115
    • 9
    Goat
    Clonality
    • 104
    • 20
    Polyclonal
    Conjugate
    • 59
    • 10
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NRF2 antibody is un-conjugated
    Application
    • 97
    • 51
    • 32
    • 29
    • 21
    • 17
    • 15
    • 15
    • 14
    • 10
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    NRF2 (aa445-458)
    Sequence
    KHSSRLEAHL TRDE
    Specificity
    This antibody is expected to recognize all reported isoforms (
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2, NP_001138884.1, NP_001138885.1.
    Isotype
    IgG
    Top Product
    Discover our top product NFE2L2 Primary Antibody
  • Application Notes

    Western Blot: Approx 70 kDa band observed in Human Lung lysates and approx 75 kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8 kDa according to NP_006155.2). This molecular weight rate

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Alternative Name
    NFE2L2 (NFE2L2 Products)
    Synonyms
    Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody
    Background
    NFE2L2, nuclear factor (erythroid-derived 2)-like 2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2, OTTHUMP00000205251, OTTHUMP00000207980, nuclear factor erythroid 2-related factor 2, nuclear factor, erythroid derived 2, like 2
    Molecular Weight
    67.8kDa according to NP_006155.2
    Gene ID
    4780, 18024, 83619
    NCBI Accession
    NP_006155, NP_001138884, NP_001138885
    Pathways
    ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
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