14-3-3 sigma/SFN antibody (AA 179-228)

Catalog No. ABIN6747165

Product Details anti-14-3-3 sigma/SFN Antibody

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Binding Specificity: AA 179-228

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, Guinea Pig, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SFN / 14-3-3 Sigma

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Rabbit, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human SFN (P31947, NP_006133). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch (100%), Hamster, Platypus (92%), Trout, Salmon, Zebrafish, Slime mold (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN / Stratifin / 14-3-3 Sigma (SFN Products)

Synonyms: YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody

Background: Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID: 2810

NCBI Accession: NP_006133

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction