POU2F1 antibody (AA 92-106)

Catalog No. ABIN793127

Product Details anti-POU2F1 Antibody

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Binding Specificity: AA 92-106

Reactivity: Human, Pig, Rabbit, Chicken, Horse, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This POU2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human POU2F1 / Oct-1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Panda, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Monkey, Marmoset, Mouse, Elephant, Bovine, Dog (93%) Platypus, Lizard (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NVQSKSNEESGDSQQ from an internal region (near N-terminus) of human POU2F1 / Oct-1 (NP_002688.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Panda, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Monkey, Marmoset, Mouse, Elephant, Bovine, Dog (93%), Platypus, Lizard (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:4000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 100kD band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kD according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POU2F1

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Alternative Name: POU2F1 / OCT1 (POU2F1 Products)

Synonyms: OCT1 antibody, OTF1 antibody, oct-1B antibody, POU2F1 antibody, 2810482H01Rik antibody, NF-A1 antibody, Oct-1 antibody, Oct1 antibody, Otf-1 antibody, Otf1 antibody, chOCT-1 antibody, oct-1 antibody, oct1 antibody, pou2f1r antibody, fc07d06 antibody, wu:fc07d06 antibody, zgc:158360 antibody, POU class 2 homeobox 1 antibody, POU domain, class 2, transcription factor 1 antibody, POU class 2 homeobox 1 S homeolog antibody, POU class 2 homeobox 1b antibody, POU2F1 antibody, Pou2f1 antibody, pou2f1.S antibody, pou2f1b antibody

Background: Name/Gene ID: POU2F1

Synonyms: POU2F1, NF-A1, OCT1, Octamer-binding protein 1, Oct-1B, Oct-1, OTF-1, OTF1, POU class 2 homeobox 1

Gene ID: 5451

NCBI Accession: NP_002688

UniProt: P14859