SLC5A5 antibody (AA 468-643)

Catalog No. ABIN863183

Product Details anti-SLC5A5 Antibody

Target: SLC5A5 (Solute Carrier Family 5 (Sodium/iodide Cotransporter), Member 5 (SLC5A5))

Binding Specificity: AA 468-643

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC5A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Antibody Array (AA)

Specificity: Detects ~97 kDa, non-glycosylated version at 68 kDa. Other minor bands associated with hNIS at 160 kDa, and degradation products at ~30 kDa, and ~15 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Mannose binding protein hNIS fusion (AA468-643)

Clone: 14F

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863183 was sufficient for detection of hNIS in 20 μg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for SLC5A5

Target: SLC5A5 (Solute Carrier Family 5 (Sodium/iodide Cotransporter), Member 5 (SLC5A5))

Alternative Name: Sodium Iodide Symporter (SLC5A5 Products)

Synonyms: Na(+)/I(-) cotransporter antibody, Na(+)/I(-) symporter antibody, Nis antibody, solute carrier family 5 member 5 antibody, Slc5a5 antibody

Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Gene ID: 6528

NCBI Accession: NP_000444

UniProt: Q92911