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SLC12A3 antibody (AA 74-95)

SLC12A3 Reactivity: Rat WB, IHC, ICC, IF, IEM Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN863201
  • Target See all SLC12A3 Antibodies
    SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
    Binding Specificity
    • 16
    • 10
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 74-95
    Reactivity
    • 34
    • 13
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 42
    • 4
    Rabbit
    Clonality
    • 42
    • 4
    Polyclonal
    Conjugate
    • 11
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SLC12A3 antibody is un-conjugated
    Application
    • 25
    • 16
    • 14
    • 14
    • 13
    • 13
    • 12
    • 10
    • 9
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)
    Specificity
    Detects ~160 kDa.
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Affinity Purified
    Immunogen
    Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
  • Application Notes
    • WB (1:1000)
    • IHC (1:200)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN863201 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Fenton, Poulsen, de la Mora Chavez, Soleimani, Dominguez Rieg, Rieg: "Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis." in: Kidney international, Vol. 92, Issue 2, pp. 397-414, (2018) (PubMed).

    Procino, Carmosino, Milano, Dal Monte, Schena, Mastrodonato, Gerbino, Bagnoli, Svelto: "β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function." in: Kidney international, Vol. 90, Issue 3, pp. 555-67, (2017) (PubMed).

    Patel-Chamberlin, Varasteh Kia, Xu, Barone, Zahedi, Soleimani: "The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150918, (2016) (PubMed).

    Gois, Canale, Luchi, Volpini, Veras, Costa, Shimizu, Seguro: "Tenofovir during pregnancy in rats: a novel pathway for programmed hypertension in the offspring." in: The Journal of antimicrobial chemotherapy, Vol. 70, Issue 4, pp. 1094-105, (2015) (PubMed).

    Wen, Yuan, Warner, Wang, Cornelius, Wang-France, Li, Boettger, Sansom: "Increased Epithelial Sodium Channel Activity Contributes to Hypertension Caused by Na+-HCO3- Cotransporter Electrogenic 2 Deficiency." in: Hypertension, Vol. 66, Issue 1, pp. 68-74, (2015) (PubMed).

    Cheng, Wu, Kortenoeven, Pisitkun, Fenton: "A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells." in: Scientific reports, Vol. 5, pp. 12829, (2015) (PubMed).

    Rosenbaek, Kortenoeven, Aroankins, Fenton: "Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13347-61, (2014) (PubMed).

    Esteva-Font, Guillén-Gómez, Diaz, Guirado, Facundo, Ars, Ballarin, Fernández-Llama: "Renal sodium transporters are increased in urinary exosomes of cyclosporine-treated kidney transplant patients." in: American journal of nephrology, Vol. 39, Issue 6, pp. 528-35, (2014) (PubMed).

    Donnelly, Needham, Snyder, Roy, Khadem, Brodsky, Subramanya: "Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis." in: The Journal of biological chemistry, Vol. 288, Issue 18, pp. 13124-35, (2013) (PubMed).

    Rosenbaek, Assentoft, Pedersen, MacAulay, Fenton: "Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC." in: The Journal of physiology, Vol. 590, Issue Pt 23, pp. 6121-39, (2012) (PubMed).

  • Target
    SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
    Alternative Name
    NCC (SLC12A3 Products)
    Synonyms
    SLC12A3 antibody, slc12a3 antibody, DKFZp469N2315 antibody, NCC antibody, NCCT antibody, TSC antibody, AI035291 antibody, solute carrier family 12 member 3 antibody, solute carrier family 12 (sodium/chloride transporter), member 3 antibody, solute carrier family 12, member 3 antibody, SLC12A3 antibody, slc12a3.2 antibody, Slc12a3 antibody
    Background
    NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
    Gene ID
    54300, 6559
    NCBI Accession
    NP_062218, NP_000330
    UniProt
    P55018, P55017
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