SIM1 antibody (Internal Region)

Catalog No. ABIN870636

Product Details anti-SIM1 Antibody

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: SIM1

Sequence: SGDRYRTEQY QS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SGDRYRTEQYQS, from the internal region of the protein sequence according to NP_005059.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in lysates of cell line HEK293 (calculated MW of 85.5 kDa according to NP_005059.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIM1

Target: SIM1 (Single-Minded Homolog 1 (SIM1))

Alternative Name: SIM1 (SIM1 Products)

Synonyms: bHLHe14 antibody, mSIM1 antibody, single-minded family bHLH transcription factor 1 antibody, single-minded homolog 1 (Drosophila) antibody, single-minded family bHLH transcription factor 1a antibody, SIM1 antibody, Sim1 antibody, sim1a antibody

Background: SIM1, single-minded homolog 1 (Drosophila), bHLHe14, OTTHUMP00000016902, class E basic helix-loop-helix protein 14, single-minded homolog 1

Molecular Weight: 85.5kDa

Gene ID: 6492, 20464, 309888

NCBI Accession: NP_005059