CYP1A1 antibody (Internal Region)

Catalog No. ABIN870663

Product Details anti-CYP1A1 Antibody

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: cytochrome P450 1A1 (mouse)

Sequence: QDRKLDENAN VQLSD

Specificity: This antibody is not expected to cross-react with the similar P450 1A2

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QDRKLDENANVQLSD, from the internal region of the protein sequence according to NP_001129531.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human and Rat Lung lysates (calculated MW of 58.2 kDa according to Human NP_000490.1 and 59.4 kDa according to Rat NP_036672.). Primary incubation was 1 hour. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP1A1

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Alternative Name: cyp1a1 (CYP1A1 Products)

Synonyms: AHH antibody, AHRR antibody, CP11 antibody, CYP1 antibody, P1-450 antibody, P450-C antibody, P450DX antibody, Cyp1a2 antibody, P450-1 antibody, cyp1a1 antibody, CYP1A1 antibody, CYPIA1 antibody, Cyp45c antibody, Cypc45c antibody, P-450MC antibody, wu:fb63b04 antibody, zfCYP1A antibody, zgc:109747 antibody, ahh antibody, ahrr antibody, cp11 antibody, cyp1 antibody, cyp1a antibody, p1-450 antibody, p450-c antibody, p450dx antibody, cytochrome P450 family 1 subfamily A member 1 antibody, cytochrome P450 1A1 antibody, cytochrome P450, family 1, subfamily a, polypeptide 1 antibody, cytochrome P450, family 1, subfamily A, polypeptide 1 antibody, cytochrome P450 family 1 subfamily D polypeptide 1 antibody, cytochrome P4501A1 antibody, polycyclic hydrocarbon-inducible cytochrome P450c antibody, cytochrome P450, family 1, subfamily A antibody, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 antibody, cytochrome P450 family 1 subfamily A member 1 L homeolog antibody, CYP1A1 antibody, CpipJ_CPIJ010542 antibody, Cyp1a1 antibody, cyp1d1 antibody, LOC100328613 antibody, cyp1a antibody, LOC102129994 antibody, cyp1a1.L antibody

Background: Cyp1a1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, Cyp1a2, P450-1, CYPIA1, aromatic compound inducible, cytochrome P450 1A1, cytochrome P450 family 1 subfamily a polypeptide 2, cytochrome P450 subfamily I, polypeptide 1, cytoc

Molecular Weight: 59.2kDa

Gene ID: 13076, 24296

NCBI Accession: NP_001129531

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha