CDC25B antibody (pSer149)

Catalog No. ABIN871399

Product Details anti-CDC25B Antibody

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Binding Specificity: pSer149

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

Purification: Affinity purified

Immunogen: Peptide sequence around phosphorylation site of pSer149 (F-R-S (p) -L-P) derived from Mouse Cdc25B. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-CDC25B antibody (ABIN871399)

Xiao, Liu, Hou, Cui, Wu, Fan, Sun, Meng, Yang, Wang, Yu: "Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs." in: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10356-66, (2011) (PubMed).

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: Cdc25B (CDC25B Products)

Synonyms: CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody

Background: Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Molecular Weight: 62 kDa

Gene ID: 12531

NCBI Accession: NP_001104

UniProt: Q9DBN8

Pathways: Cell Division Cycle, M Phase, Autophagy