Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous levels of PKCb only when phosphorylated at threonine 641.
Purification
Affinity purified
Immunogen
Peptide sequence around phosphorylation site of pThr641 (E-L-T (p) -P-T) derived from Human PKCb Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC-B antibody, Prkcb antibody, prkcb1 antibody, zgc:63591 antibody, PKC antibody, PRKCB_tv2 antibody, Pkc53E antibody, prkcb1l antibody, zgc:64063 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, protein kinase C, beta b antibody, protein kinase C, beta a antibody, PRKCB antibody, Prkcb antibody, prkcbb antibody, prkcba antibody
Background
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.