KRT12 antibody (AA 151-250)

Catalog No. ABIN872955

Product Details anti-KRT12 Antibody

Target: KRT12 (Keratin 12 (KRT12))

Binding Specificity: AA 151-250

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK12/Cytokeratin 12

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for KRT12

Target: KRT12 (Keratin 12 (KRT12))

Alternative Name: CK12 (KRT12 Products)

Background:

Synonyms: 65 kDa cytokeratin, CK 12, CK 3, CK12, CK3, Cytokeratin 12, Cytokeratin 3, K12, K3, keratin 12 Meesmann corneal dystrophy, Keratin 12, Keratin 3, Keratin, type I cytoskeletal 12, K1C12_HUMAN, Keratin, type II cytoskeletal 3, KRT12, KRT3.

Background: Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD)

Molecular Weight: 54kDa

Gene ID: 3859