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Collagen IV antibody (AA 1571-1669) (Alexa Fluor 488)

COL4 Reactivity: Human, Mouse, Rat, Pig WB, FACS, IF (p), IF (cc) Host: Rabbit Polyclonal Alexa Fluor 488
Catalog No. ABIN891716
  • Target See all Collagen IV (COL4) Antibodies
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Binding Specificity
    • 16
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1571-1669
    Reactivity
    • 90
    • 42
    • 40
    • 22
    • 16
    • 11
    • 5
    • 2
    Human, Mouse, Rat, Pig
    Host
    • 59
    • 27
    • 13
    Rabbit
    Clonality
    • 71
    • 28
    Polyclonal
    Conjugate
    • 50
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This Collagen IV antibody is conjugated to Alexa Fluor 488
    Application
    • 61
    • 50
    • 31
    • 28
    • 27
    • 27
    • 25
    • 25
    • 20
    • 10
    • 9
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
    Cross-Reactivity
    Human, Mouse, Pig, Rat
    Predicted Reactivity
    Dog,Cow,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain
    Isotype
    IgG
    Top Product
    Discover our top product COL4 Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Belair, Wolf, Wood, Ren, Grindstaff, Padgett, Swank, MacMillan, Fisher, Winnik, Abbott: "Engineering human cell spheroids to model embryonic tissue fusion in vitro." in: PLoS ONE, Vol. 12, Issue 9, pp. e0184155, (2017) (PubMed).

  • Target
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Alternative Name
    Collagen 4 (COL4 Products)
    Synonyms
    collagen, type IV antibody, Let-2 antibody
    Background

    Synonyms: ICH, HANAC, POREN1, arresten, Collagen alpha-1(IV) chain, COL4A1

    Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

    Gene ID
    1282
    UniProt
    P02462
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