PUS10 antibody (AA 101-200) (HRP)

Catalog No. ABIN912605

Product Details anti-PUS10 Antibody (HRP)

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUS10 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUS10/CCDC139

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PUS10

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Alternative Name: CCDC139 (PUS10 Products)

Synonyms: CCDC139 antibody, DOBI antibody, 2810013G11Rik antibody, 4933435A13Rik antibody, AU014648 antibody, C77560 antibody, Ccdc139 antibody, RGD1306402 antibody, pseudouridylate synthase 10 antibody, PUS10 antibody, Pus10 antibody

Background:

Synonyms: CCDC139, Coiled coil domain containing protein 139, DOBI, FLJ32312, MGC126729, Putative tRNA pseudouridine synthase Pus10, tRNA pseudouridine 55 synthase, tRNA uridine isomerase, PUS10_HUMAN.

Background: Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA. It is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones which facilitate the correct folding and assembly of tRNAs.