PKC iota antibody (Middle Region)

Catalog No. ABIN928851

Product Details anti-PKC iota Antibody

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: PRKCI antibody was raised in rabbit using the middle region of PRKCI as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PRKCI Blocking Peptide, catalog no. 33R-9357, is also available for use as a blocking control in assays to test for specificity of this PRKCI antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PRKCI (PRKCI Products)

Background: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. Synonyms: Polyclonal PRKCI antibody, Anti-PRKCI antibody, protein kinase C, iota antibody, DXS1179E antibody, MGC26534 antibody, PKCI antibody, nPKC-iota antibody.

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation