PSMF1 antibody (Middle Region)

Catalog No. ABIN929149

Product Details anti-PSMF1 Antibody

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMF1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: PSMF1 antibody was raised in rabbit using the middle region of PSMF1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PSMF1 Blocking Peptide, catalog no. 33R-2238, is also available for use as a blocking control in assays to test for specificity of this PSMF1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for PSMF1

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Alternative Name: PSMF1 (PSMF1 Products)

Synonyms: PI31 antibody, AW048666 antibody, BC012260 antibody, zgc:92785 antibody, proteasome inhibitor subunit 1 antibody, proteasome (prosome, macropain) inhibitor subunit 1 antibody, PSMF1 antibody, Psmf1 antibody, psmf1 antibody

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. Synonyms: Polyclonal PSMF1 antibody, Anti-PSMF1 antibody, proteasome, prosome, macropain inhibitor subunit 1, PI31 antibody, PI31 antibody.

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA