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BRAF antibody
BRAF
Reactivity: Human
WB, ELISA, IHC
Host: Mouse
Monoclonal
1H12F1-1H12G10-1F12F11C9
unconjugated
Product Details anti-BRAF Antibody
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Target
See all BRAF Antibodies
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Reactivity
All reactivities for BRAF antibodies
Human
Host
All hosts for BRAF antibodies
Mouse
Clonality
All clonalities for BRAF antibodies
Monoclonal
Conjugate
All conjugates for BRAF antibodies
This BRAF antibody is un-conjugated
Application
All applications for BRAF antibodies
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Immunogen
BRAF antibody was raised in Mouse using a purified recombinant fragment of BRAF expressed in E. coli as the immunogen.
Clone
1H12F1-1H12G10-1F12F11C9
Isotype
IgG1
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Discover our top product BRAF Primary Antibody
Alternatives
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Application Details
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Application Notes
ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
Lot specific
Buffer
Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Target Details for BRAF
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Target
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Alternative Name
BRAF (BRAF Products )
Synonyms
B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody
Background
BRAF is one of the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylation sites. B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Synonyms: Monoclonal BRAF antibody, Anti-BRAF antibody, V-raf murine sarcoma viral oncogene homolog B1 antibody, BRAF1 antibody, RAFB1 antibody, B-RAF1 antibody, B RAF1 antibody.
Pathways
MAPK Signaling , RTK Signaling , Neurotrophin Signaling Pathway , Ribonucleoprotein Complex Subunit Organization , Hepatitis C , Autophagy
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