CPE antibody

Catalog No. ABIN933894

Product Details anti-CPE Antibody

Target: CPE (Carboxypeptidase E (CPE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Sterility: Filtered

Immunogen: Carboxypeptidase E antibody was raised in mouse using purified, NSO-derived, recombinant human CPE as the immunogen.

Clone: 420709-M8100701

Isotype: IgG2a

Application Details

Application Notes: ELISA: 0.5-1.0 µg/mL, IP: 25 µg/mL, WB: 1-2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 0.5 mg/mL.

Concentration: Lot specific

Buffer: Supplied lyophilized from a 0.2 µm filtered solution in PBS, with 5 % Trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for CPE

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase E (CPE Products)

Synonyms: CPE antibody, CPH antibody, Cph-1 antibody, Cph1 antibody, R74677 antibody, fat antibody, CARBE antibody, Cph antibody, si:ch211-198g9.2 antibody, zgc:85981 antibody, carboxypeptidase E antibody, carboxypeptidase E L homeolog antibody, CPE antibody, cpe antibody, Cpe antibody, cpe.L antibody

Background: Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters. CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance. The CPE precursor includes a signal peptide (aa 1 - 25), a propeptide (aa 26 - 42) and a mature chain (aa 43 - 476). The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken. Synonyms: Monoclonal Carboxypeptidase E antibody, Anti-Carboxypeptidase E antibody.

Pathways: Peptide Hormone Metabolism, Synaptic Membrane