ATP1B1 antibody (Subunit beta)

Catalog No. ABIN5684017

Product Details anti-ATP1B1 Antibody

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Binding Specificity: Subunit beta

Reactivity: Rat, Arabidopsis thaliana, Pig, Spinach, Zea mays subsp. parviglumis (Balsas teosinte), Soybean, Salmon, Oryza sativa, Nicotiana tabacum, Nicotiana benthamiana, Chlamydomonas reinhardtii, Chicken, Barley, Bacillus cereus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Blue-native PAGE (BN PAGE)

Specificity: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.

Cross-Reactivity (Details): No cross-reactivity with: archeal V-type ATP synthase

Characteristics: Expected / apparent Molecular Weight of the Antigene: 53.9kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Application Details

Application Notes: Recommended Dilution: 1 : 2000 - 1 : 5 000 with standard ECL (WB), 1 : 5000 (BN-PAGE).

Comment:

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 10 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-ATP1B1 antibody (ABIN5684017)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Berto, DAdamo, Bergantino, Vallese, Giacometti, Costantini: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." in: Biochemical and biophysical research communications, (2011) (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." in: Amino acids, (2010) (PubMed).

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: AtpB (ATP1B1 Products)

Synonyms: ATP1B antibody, Atp4b antibody, Atpb antibody, Atpb-1 antibody, NKbeta1 antibody, ATPBS antibody, atp1b1 antibody, atp1b1a antibody, cb710 antibody, ATPase Na+/K+ transporting subunit beta 1 antibody, ATPase, Na+/K+ transporting, beta 1 polypeptide antibody, ATPase beta chain antibody, ATP synthase CF1 beta subunit antibody, ATPase Na+/K+ transporting subunit beta 1 S homeolog antibody, ATPase, Na+/K+ transporting, beta 1b polypeptide antibody, ATP1B1 antibody, Atp1b1 antibody, atpB antibody, atp1b1.S antibody, atp1b1b antibody

Background: AGI Code: At5g08670, ATCG00480
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Molecular Weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

UniProt: P83483, A8IQU3, P19366, P06541

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome