CD235ab antibody (N-Term)
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- Target See all CD235ab (CD235a + CD235b) products
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- Binding Specificity
- N-Term
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD235ab antibody is un-conjugated
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Aggregation (AGG)
- Specificity
- The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Synthetic peptide (Human, N-terminal)
- Clone
- HIR2
- Isotype
- IgG2b
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anti-Glycophorin A + B (CD235a + CD235b) (N-Term) antibody (FITC)
CD235a + CD235b Reactivity: Human FACS Host: Mouse Monoclonal HIR2 FITC
anti-Glycophorin A + B (CD235a + CD235b) (N-Term) antibody (PE)CD235a + CD235b Reactivity: Human FACS Host: Mouse Monoclonal HIR2 PE
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- Application Notes
- Flow cytometry: Recommended dilution: 1-4 μg/mL
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- Alternative Name
- CD235ab (CD235a + CD235b Products)
- Background
- CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group
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