FAS antibody (Fas (TNF Receptor Superfamily, Member 6))

Details for Product anti-FAS Antibody No. ABIN94250
Antigen
Reactivity
Human
604
101
90
4
3
2
1
1
1
Host
Mouse
353
319
7
4
4
3
Clonality (Clone)
Monoclonal ()
Conjugate
This FAS antibody is un-conjugated
67
56
42
26
15
8
8
8
7
7
7
7
4
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
303
284
198
149
79
67
66
60
52
37
19
16
10
8
7
6
4
4
3
3
2
2
1
1
1
1
Options
Immunogen HUT-78 human T cell lymphoma cells
Clone UT-1
Isotype IgM
Specificity The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Antigen
Alternative Name CD95 / Fas (FAS Antibody Abstract)
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free HEPES buffered saline (HBS), approx. pH 7.0, 0.2 um filter sterilized.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody (ABIN94250) Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodi...
Product cited in: Matsuyama, Mitsuyama, Ono, Shirahama, Higashimoto, Osame, Arimura: "Discoidin domain receptor 1 contributes to eosinophil survival in an NF-kappaB-dependent manner in Churg-Strauss syndrome." in: Blood, Vol. 109, Issue 1, pp. 22-30, 2006 (PubMed).