HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)

Details for Product No. ABIN94363
Antigen
Reactivity
Human
322
9
8
5
1
1
1
1
Host
Mouse
219
81
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
43
40
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
184
170
170
110
63
47
36
33
22
18
13
13
8
3
2
2
2
1
1
Options
Immunogen HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Clone 87G
Isotype IgG2a
Specificity The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Human
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule on the cell surface, and for ELISA. Suggested working dilution for Flow Cytometry is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin) (ABIN94363) Analysis of cytolytical activity Analysis of cytolytical activity of human polyclonal...
Product cited in: LeMaoult, Caumartin, Daouya, Favier, Le Rond, Gonzalez, Carosella: "Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells." in: Blood, Vol. 109, Issue 5, pp. 2040-8, 2007 (PubMed).

Shobu, Sageshima, Tokui, Omura, Saito, Nagatsuka, Nakanishi, Hayashi, Hatake, Ishitani: "The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation." in: Journal of reproductive immunology, Vol. 72, Issue 1-2, pp. 18-32, 2006 (PubMed).

Rouas-Freiss, Moreau, Ferrone, Carosella: "HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism?" in: Cancer research, Vol. 65, Issue 22, pp. 10139-44, 2005 (PubMed).

Hackmon, Hallak, Krup, Weitzman, Sheiner, Kaplan, Weinstein: "HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy." in: Fetal diagnosis and therapy, Vol. 19, Issue 5, pp. 404-9, 2004 (PubMed).

Ishitani, Sageshima, Lee, Dorofeeva, Hatake, Marquardt, Geraghty: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Poláková, Bandzuchová, Hofmeister, Weiss, Hutter, Russ: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Poláková, Krcová, Kuba, Russ: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Sageshima, Ishitani, Omura, Akasaki, Umekage, Katabuchi, Okamura, Hatake: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 49, Issue 3, pp. 174-82, 2003 (PubMed).

Wiendl, Mitsdoerffer, Hofmeister, Wischhusen, Weiss, Dichgans, Lochmuller, Hohlfeld, Melms, Weller: "The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy." in: Brain : a journal of neurology, Vol. 126, Issue Pt 1, pp. 176-85, 2002 (PubMed).

Riteau, Menier, Khalil-Daher, Martinozzi, Pla, Dausset, Carosella, Rouas-Freiss: "HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition." in: International immunology, Vol. 13, Issue 2, pp. 193-201, 2001 (PubMed).