Mouse anti-Cow (Bovine) IgG (Light Chain) Antibody

Details for Product No. ABIN94401, Supplier: Log in to see
Antigen
Epitope
Light Chain
4680
2535
1881
1066
1005
859
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Cow (Bovine)
2848
2736
2292
1675
1433
788
613
441
380
379
358
339
291
290
208
138
135
125
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
Host
Mouse
6701
4830
1796
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Fraction of Bovine immunoglobulin.
Clone IVA285-1
Isotype IgG1
Specificity The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Research Area Immunology, Secondary Antibodies
Application Notes Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG (Light Chain) antibody (ABIN94401) Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
Product cited in: Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).