Kinesin antibody (AA 331-906)

Catalog No. ABIN94498

Product Details anti-Kinesin Antibody

Target: Kinesin

Binding Specificity: AA 331-906

Reactivity: Human, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kinesin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: The polyclonal antibody detects total level of endogenous kinesin protein (intracellular antigen).

Cross-Reactivity (Details): Human, Mouse, Porcine, Other not tested

Purification: Purified by ligand affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Kinesin antibody (ABIN94498)

Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).

Target Details for Kinesin

Target: Kinesin

Abstract: Kinesin Products

Synonyms: kinesin antibody, putative kinesin antibody, Tc00.1047053510323.120 antibody, Tc00.1047053508153.200 antibody, LmjF29.2390 antibody, LINJ_29_2500 antibody, LBRM_29_2360 antibody

Background: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KIN