AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 460-480)

Details for Product anti-AKT1 Antibody No. ABIN94784
Antigen
Epitope
AA 460-480, C-Term, pSer473
173
84
78
67
50
45
45
30
26
25
24
24
19
18
17
17
14
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9
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4
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3
3
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2
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2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
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1
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Reactivity
Human
1245
705
592
52
52
48
33
33
30
28
6
6
5
4
4
3
3
2
1
1
1
1
Host
Rabbit
1047
233
9
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
62
37
34
32
29
26
19
19
19
18
18
18
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), ELISA, Western Blotting (WB)
1061
594
563
204
177
148
116
61
55
33
26
18
13
11
8
7
5
3
3
1
1
1
1
1
1
Options
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Antiserum
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at  Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phos-phorylated, AKT dissociates from the membrane and phos-phorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes This antibody is phospho specific for pS473 and is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections), immunofluorescence and ELISA. By immunoblot a single band of the expected apparent molecular weight is observed.  For immunohistochemistry no pre-treatment of sample is required.
Restrictions For Research Use only
Format Liquid
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term), (pSer473) antibody (ABIN94784) Immunohistochemistry of Rabbit anti-AKT pS473 antibody. Tissue: human breast carcinom...
Image no. 2 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term), (pSer473) antibody (ABIN94784) Western Blot of Rabbit anti-AKT pS473 antibody. Lane 1: AKT1 Recombinant Protein . La...
Image no. 3 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term), (pSer473) antibody (ABIN94784) Immunohistochemistry. This image is a higher magnification of the breast tumor image ...
Image no. 4 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term), (pSer473) antibody (ABIN94784) Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinom...
Product cited in: Henle, Wang, Liang, Wu, Poo, Henley: "Asymmetric PI(3,4,5)P3 and Akt signaling mediates chemotaxis of axonal growth cones." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 19, pp. 7016-27, 2011 (PubMed).